Advanced Gene Editing
Targeted genome editing has emerged as a crucial tool in research, bridging the gap between discovery and therapeutic applications. This technology allows scientists to selectively disrupt, recover, repress, or activate gene expression, offering an unparalleled ability to explore and manipulate genetic mechanisms that govern biological processes. The applications of genome engineering are vast, ranging from curing genetic and developmental disorders to the creation of disease-resistant crops, revolutionizing both medical and agricultural fields.
Overview
CRISPR – Accurate, Efficient Gene Editing
Gene editing is a specific and targeted change to a DNA sequence and involves the addition, removal, or modification of the DNA. The CRISPR-Cas system (evolved in microbes as a defense mechanism) is the basis for a class of gene-editing tools that are enabling advances from health and diagnostics to agriculture and energy. Using CRISPR, researchers have the power to target a specific gene, gene family, or even screen an entire genome.
CRISPRi and CRISPRa – Powerful Gene Inhibition and Activation
CRISPRi (CRISPR interference) and CRISPRa (CRISPR activation) deliver highly efficient silencing and activation of genes, respectively, without altering the underlying DNA sequence. When employed in large-scale LOF (loss-of-function) and GOF (gain-of-function) screens, researchers are able to identify unique, yet functionally related, gene pathways that are often missed with other methods.
CRISPRi Whole Genome and Non-Coding Libraries
Sigma-Aldrich® Advanced Genomics offers a complete suite of optimized CRISPRi and CRISPRa libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.
Connect with a Sigma-Aldrich® Advanced Genomics Expert
Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert to get guidance on the best solutions for your research.