Gene Expression & Silencing
Modulating gene expression is a vital technique in research to understand and manipulate the complex genetic networks that control biological processes. This approach is particularly valuable in identifying potential therapeutics for diseases such as cancer, neurodegenerative disorders, and developmental conditions. Various technologies, including RNA interference (RNAi), CRISPR inhibition (CRISPRi), and CRISPR activation (CRISPRa), are employed for precise regulation of gene expression in cells and tissues. RNAi, utilizing siRNA duplexes, provides targeted and potent inhibition of gene expression. Meanwhile, CRISPR technologies offer researchers the flexibility to regulate genes by either silencing or activating them, enabling a broad range of genetic modifications. SigmaAldrich® Advanced Genomics leads in providing cutting-edge tools and products for these gene modulation strategies, enhancing the ability of researchers to explore gene functions and develop new treatments.
Overview
RNAi - Gene Modulation to Illuminate Genetic Pathways
Functional genomics screens are an integral part of discovery research, allowing you to unravel the mechanisms that belie biological systems, pathways, and diseases. Effective interrogation of these complex molecular interaction networks can be accomplished using shRNA libraries to deliver reliable, efficient, and long-term knockdown.
Sigma-Aldrich® Advanced Genomics is the exclusive provider of the TRC1.5 and TRC2 collections from The RNAi Consortium (TRC). These lentivirus-based shRNA libraries deliver unparalleled coverage and are available in multiple formats that include whole-genome, individual RNAi clones/vectors, and gene family sets.
Explore shRNA CLONES & LIBRARIES
CRISPRi and CRISPRa - Powerful Gene Inhibition and Activation
CRISPRi (CRISPR interference) and CRISPRa (CRISPR activation) deliver highly efficient silencing and activation of genes, respectively, without altering the underlying DNA sequence. When employed in large scale LOF (loss-of-function) and GOF (gain-of-function) screens, researchers are able to identify unique, yet functionally related, gene pathways that are often missed with other methods.
Sigma-Aldrich® Advanced Genomics offers a complete suite of optimized CRISPRi and CRISPRa libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.
CRISPRi Whole Genome and Non-Coding Libraries
CRISPRa Whole Genome SAM Libraries