Description

• Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, including to cleave Asn-Gly peptide bonds and to deprotect (expose) sulfhydryls for conjugation following modification with SATA and similar reagents
• Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation
• Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS
• Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base
• Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine-HCl
• Highlights: Exposes protected sulfhydryl groups on SATA-modified molecules Cleaves carbonyl-containing crosslinkers such as EGS Reagent Properties: Chemical name: Hydroxylamine Hydrochloride Chemical formula: NH 2 OH·HCl Molecular weight: 69.49 CAS Number: 5470-11-1 Form: White crystals, free of foreign material Solubility: 10%