Description

• General description: Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes.Together with the companion product Phospho-L-Tyrosine disodium Salt disodium salt, the new modified amino acid S-Sulfocysteine sodium salt can be used as replacement for cysteine to generate highly concentrated, neutral pH feeds. Both modified amino acids eliminate the need for alkaline feeds, which are normally applied to ensure solubility and stability of the unmodified amino acids tyrosine and cysteine.
• Features and Benefits: Reduced complexity in fed-batch processHigh concentrations of modified cysteine in main feeds at neutral pHPrevention of caustic shocks in the bioreactor due to high pH feedsMore convenient preparation process with less contamination risksReduction of recombinant protein fragmentation and decrease in trisulfide bond contentHigher feed stability at room temperature
• Linkage: WebinarTitle: Simplification of Fed-Batch Processes Using Modified Amino Acids
• Legal Information: Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany