PCR-Based DNA Profiling Standard

Description

• General description: This PCR DNA profiling standard comprises well-characterized human deoxyribonucleic acid (DNA) in two forms, genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto Flinders technology associates (FTA) paper (Component E). The components are derived from human buffy coat white blood cells and labeled A, B, and C. Component E is a cell line obtained from American Type Culture Collection. Component A contains 55 µL of single-source female genomic DNA in TE-4 buffer Components B and C contain 55 µL each of single-source male genomic DNA in TE-4 buffer Component D contains 55 µL of genomic DNA in TE-4 buffer which is a mix of components A and C in the ratio of 3:1 Component E contains single-source female cells spotted on two 6 mm punches of FTA paper such that each punch can hold approximately 7.5 × 104 cells A unit of this standard reference material (SRM) is composed of one vial of each of the five components packaged together in a single box.SRM 2391d_CertSRM 2391d_SDS
• Application: This PCR DNA profiling standard is intended for use in the standardization of forensic and paternity quality assurance procedures for polymerase chain reaction (PCR) based genetic testing, for instructional law enforcement or non-clinical research purposes, and quality assurance when assigning values to in-house control materials.
• Features and Benefits: The use of this material will provide confidence to the assignment of values to in-house prepared control materials for PCR DNA Profiling
• Other Notes: Details on expiration, storage, safety, usage, and source are provided in the NIST certificateInformation on biomaterials, disposal, and transport is available in the SDSThe certified values for NIST2391d are derived from a combination of Capillary Electrophoresis (CE) and Next Generation Sequencing (NGS)-based characterizations that allowed for the counting and direct sequencing of short tandem repeats (STRs) at a locus.
• Preparation Note: Before opening vials of components A, B, C, and D must be vortexed and centrifugedPrior PCR amplification component E, cells on the FTA should be washed to remove PCR inhibitors and salts
• Legal Information: NIST is a registered trademark of National Institute of Standards and Technology