01-675-859
Mouse IgG2a, FITC, clone: ZX4, Isotype Control, Invitrogen™
Manufacturer: Thermo Scientific
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Antigen
Mouse IgG2a
Clone
ZX4
Gene Alias
IgG2, IgG2a
Immunogen
White blood cells
Quantity
100 Tests
Primary or Secondary
Isotype Control (Primary)
Content And Storage
Store at 4°C, do not freeze
Isotype
IgG2a κ
Applications
Control, Flow Cytometry
Conjugate
FITC
Host Species
Mouse
Purification Method
Protein A/G
Regulatory Status
RUO
Target Species
Not Applicable
Form
Liquid
Related Products
Description
- The isotype of a primary antibody and the application it is being used in can result in background staining
- Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
- Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
- While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
- Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
- If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody
- SA1-12080 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.
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Antigen: Mouse IgG2a
Clone: ZX4
Gene Alias: IgG2, IgG2a
Immunogen: White blood cells
Quantity: 100 Tests
Primary or Secondary: Isotype Control (Primary)
Content And Storage: Store at 4°C, do not freeze
Isotype: IgG2a κ
Applications: Control, Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Purification Method: Protein A/G
Regulatory Status: RUO
Target Species: Not Applicable
Form: Liquid
Antigen:
Mouse IgG2a
Clone:
ZX4
Gene Alias:
IgG2, IgG2a
Immunogen:
White blood cells
Quantity:
100 Tests
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
Store at 4°C, do not freeze
Isotype:
IgG2a κ
Applications:
Control, Flow Cytometry
Conjugate:
FITC
Host Species:
Mouse
Purification Method:
Protein A/G
Regulatory Status:
RUO
Target Species:
Not Applicable
Form:
Liquid
Antigen: Mouse IgG1
Clone: ZX3
Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
Immunogen: White blood cells.
Quantity: 100 μg
Primary or Secondary: Isotype Control (Primary)
Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
Isotype: IgG1
Applications: Control, Flow Cytometry
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein A/G
Regulatory Status: RUO
Target Species: Not Applicable
Form: Liquid
Antigen:
Mouse IgG1
Clone:
ZX3
Gene Alias:
IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
Immunogen:
White blood cells.
Quantity:
100 μg
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
-20° C, Avoid Freeze/Thaw Cycles
Isotype:
IgG1
Applications:
Control, Flow Cytometry
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein A/G
Regulatory Status:
RUO
Target Species:
Not Applicable
Form:
Liquid
Antigen: Mouse IgG1
Clone: ZX3
Gene Alias: IgG1
Immunogen: White blood cells.
Quantity: 100 Tests
Primary or Secondary: Isotype Control (Primary)
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Isotype: IgG1 κ
Applications: Control, Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Purification Method: Protein A/G
Regulatory Status: RUO
Target Species: Not Applicable
Form: Liquid
Antigen:
Mouse IgG1
Clone:
ZX3
Gene Alias:
IgG1
Immunogen:
White blood cells.
Quantity:
100 Tests
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Isotype:
IgG1 κ
Applications:
Control, Flow Cytometry
Conjugate:
FITC
Host Species:
Mouse
Purification Method:
Protein A/G
Regulatory Status:
RUO
Target Species:
Not Applicable
Form:
Liquid
Antigen: CD4
Clone: MEM-115
Gene Alias: Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen
Immunogen: Human thymocytes and T lymphocytes.
Quantity: 100 μg
Primary or Secondary: Primary
Content And Storage: 4° C, do not freeze
Isotype: IgG2a
Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein A
Regulatory Status: RUO
Target Species: Human
Form: Liquid
Antigen:
CD4
Clone:
MEM-115
Gene Alias:
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen
Immunogen:
Human thymocytes and T lymphocytes.
Quantity:
100 μg
Primary or Secondary:
Primary
Content And Storage:
4° C, do not freeze
Isotype:
IgG2a
Applications:
Flow Cytometry, Functional Assay, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein A
Regulatory Status:
RUO
Target Species:
Human
Form:
Liquid