Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine5, eBioscience™, Invitrogen™

Description

• Description: This is a monoclonal mouse IgG2a, kappa antibody
• It is used as an isotype control for mouse IgG2a antibodies
• Applications Reported: PE-Cy5 Mouse IgG2a, K Iso Cntrl has been reported for use in flow cytometric analysis
• Applications Tested: This mouse IgG2a isotype control antibody is offered in 2 formats: - μg size: This can be used at the same concentration as the experimental antibody
• - test size: has been pre-titrated and tested by staining and flow cytometric analysis
• This can be used at test size: 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.