Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: This is a monoclonal mouse IgG2a, kappa antibody
• It is used as an isotype control for mouse IgG2a antibodies
• Applications Reported: This mouse IgG2a isotype control has been reported for use in bioassays and cell surface and intracellular flow cytometric analysis
• Applications Tested: This mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells
• It should be used at the same concentration as the experimental antibody
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.