Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 480, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG1 kappa is useful as an isotype control immunoglobulin
• Applications Reported: This eBRG1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This eBRG1 antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes
• Use at the same concentration as the experimental antibody
• Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation
• Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
• Powered by Sirigen.™ Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
• These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
• Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
• It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
• Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
• With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.