Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control
• Applications Reported: This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemcial staining, immunoprecipitation and immunoblotting (WB)
• Applications Tested: This Mouse IgG1 isotype control has been tested by flow cytometric analysis of human peripheral leukocytes and can be used at the same concentration as the experimental antibody
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.