Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: APC-eFluor™ 780 Rat IgG2b Isotype Control (APC-Alexa Fluor™ 750 replacement) has been reported for use in flow cytometric analysis
• Applications Tested: Rat IgG2b K Isotype Control APC-eFluor™ 780 has been tested by flow cytometric analysis of mouse splenocytes
• Use isotype control at the same concentration as experimental antibody
• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Light sensitivity: This tandem is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies
• IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization
• Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.