Mouse IgG3 Isotype Control (B10), FITC, eBioscience™, Invitrogen™

Description

• Description: The mouse IgG3 lambda is useful as an isotype control immunoglobulin
• Applications Reported: This mouse IgG3 isotype control has been reported for use in flow cytometric analysis and ELISA
• Applications Tested: This mouse IgG3 isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 10 μL per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgG3 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the least abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG3 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG3 is less commonly used in research and diagnostic applications compared to other IgG subtypes, but it is still used in some applications such as ELISA and immunohistochemistry.