Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 615, eBioscience™, Invitrogen™

Description

• Description: This is a monoclonal mouse IgG2a antibody
• It is used as an isotype control for mouse IgG2a antibodies
• Applications Reported: This eBM2a antibody has been reported for use in immunohistochemical staining and immunocytochemistry
• Applications Tested: This eBM2a antibody has been tested by immunocytochemistry and immunohistochemical staining
• Use isotype control at the same concentration as experimental antibody
• This product has not been validated for flow cytometric analysis
• Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75)
• A standard Alexa Fluor® 594 filter is acceptable
• Excitation: 595 nm; Emission: 615 nm
• Filtration: 0.2 μm post-manufacturing filtered
• Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.