Syrian Hamster IgG Isotype Control, Alexa Fluor™ 561, eBioscience™, Invitrogen™

Description

• Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum
• Applications Reported: This isotype control antibody has been reported for use in flow cytometric analysis
• Our internal testing shows that Alexa Fluor 561 non-specifically stains B cells in Swiss Webster and SJL mice
• Non-specific staining has not been observed in BALB/c or C57BL/6 mice
• Other strains have not been tested
• See the Antibody Testing Data for an example of this strain-dependent difference
• Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody
• Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610
• Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.