Rat IgG1 kappa Isotype Control (eBRG1), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin
• Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and ELISA
• Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions
• It should be used at the same concentration as the experimental antibody
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
• These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
• Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
• It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
• Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
• With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.