Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: This is a monoclonal mouse IgG2a, kappa antibody
• It is used as an isotype control for mouse IgG2a antibodies
• Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis
• Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells
• This should be used at the same concentration as experimental antibody
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
• It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
• Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
• It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
• Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
• It is also used in therapeutic applications, such as immunotherapy and vaccine development.