Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin
• Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA
• Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at the same concentration as the experimental antibody
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
• It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
• Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
• It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.