7069881

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Isotype Control

Manufacturer: Fischer Scientific

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Antigen

Armenian Hamster IgG

Concentration

1 mg/mL

Conjugate

Functional Grade

Host Species

Armenian Hamster

Target Species

Not Applicable

Gene Alias

IgG; Immunoglobulin G; ImmunoglobulinG

Primary or Secondary

Isotype Control (Primary)

Content And Storage

4° C

Clone

eBio299Arm

Applications

Control, Flow Cytometry, Functional Assay

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with no preservative; pH 7.2

Isotype

IgG

Purification Method

Affinity chromatography

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Description

  • Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin
  • Applications Reported: The eBio299Arm antibody has been reported for use as an isotype control for flow cytometry
  • Applications Tested: This eBio299Arm antibody has been tested by LAL assay to determine endotoxin level and by immunofluorescent staining and flow cytometric analysis of mouse splenocytes and normal human peripheral blood
  • Storage and handling: Use in a sterile environment
  • Filtration: 0.2 μm post-manufacturing filtered
  • Purity: Greater than 90%, as determined by SDS-PAGE
  • Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
  • Aggregation: Less than 10%, as determined by HPLC
  • The isotype of a primary antibody and the application it is being used in can result in background staining
  • Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
  • Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
  • While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
  • Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
  • If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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7069881

Armenian Hamster Isotype Control...


Antigen:
Armenian Hamster IgG

Concentration:
1 mg/mL

Conjugate:
Functional Grade

Host Species:
Armenian Hamster

Target Species:
Not Applicable

Gene Alias:
IgG; Immunoglobulin G; ImmunoglobulinG

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C

Clone:
eBio299Arm

Applications:
Control, Flow Cytometry, Functional Assay

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.2

Isotype:
IgG

Purification Method:
Affinity chromatography

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Mouse Monoclonal Antibody...


Antigen:
CD85k (ILT3)

Concentration:
1 mg/mL

Conjugate:
Functional Grade

Host Species:
Mouse

Target Species:
Human

Gene Alias:
CD85 antigen-like family member K; CD85K; glycoprotein 49 B; glycoprotein 49b; gp49; Gp49b; GP49B2; HM18; ILT3; ILT-3; Immunoglobulin-like transcript 3; leucocyte Ig-like receptor B4; leukocyte immunoglobulin like receptor B4; leukocyte immunoglobulin-like receptor 5; Leukocyte immunoglobulin-like receptor subfamily B member 4; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4; leukocyte immunoglobulin-like receptor, subfamily B, member 4; leukocyte surface receptor; Lilrb4; LIR5; LIR-5; mast cell surface glycoprotein Gp49B; Monocyte inhibitory receptor HM18

Primary or Secondary:
Primary

Content And Storage:
4° C

Clone:
ZM4.1

Applications:
Flow Cytometry, Functional Assay, Neutralization

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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Mouse Monoclonal Antibody...


Antigen:
IL-23 p19

Concentration:
1 mg/mL

Conjugate:
Functional Grade

Host Species:
Mouse

Target Species:
Human

Gene Alias:
il 23; IL 23 A; IL12B; Il-12b; Il12p40; Il-12p40; il23; IL-23; IL-23 subunit alpha; IL23A; IL-23A; IL-23-A; IL23P19; IL-23p19; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; P19; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798

Primary or Secondary:
Primary

Content And Storage:
4° C

Clone:
HNU2319

Applications:
Functional Assay, Neutralization

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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Rat Monoclonal Antibody...


Antigen:
MHC Class II (I-A/I-E)

Concentration:
1 mg/mL

Conjugate:
Functional Grade

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1

Primary or Secondary:
Primary

Content And Storage:
4° C

Clone:
M5/114.15.2

Applications:
Flow Cytometry, Functional Assay, Neutralization

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with no preservative; pH 7.2

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography