Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: This eB149/10H5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eB149/10H5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes
• Use at the same concentration as the experimental antibody
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies
• IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization
• Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.