7090433
Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™, Invitrogen™
Armenian Hamster Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Armenian Hamster IgG
Concentration
0.2 mg/mL
Conjugate
PE-Cyanine7
Host Species
Armenian Hamster
Target Species
Not Applicable
Gene Alias
IgG; Immunoglobulin G; ImmunoglobulinG
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
eBio299Arm
Applications
Control, Flow Cytometry
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Isotype
IgG
Purification Method
Affinity chromatography
Related Products
Description
- Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin
- Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis
- Isotype control should be used at the same concentration as experimental antibody
- Light sensitivity: This tandem dye is sensitive photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
- 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- The isotype of a primary antibody and the application it is being used in can result in background staining
- Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
- Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
- While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
- Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
- If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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Antigen: Armenian Hamster IgG
Concentration: 0.2 mg/mL
Conjugate: PE-Cyanine7
Host Species: Armenian Hamster
Target Species: Not Applicable
Gene Alias: IgG; Immunoglobulin G; ImmunoglobulinG
Primary or Secondary: Isotype Control (Primary)
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: eBio299Arm
Applications: Control, Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG
Purification Method: Affinity chromatography
Antigen:
Armenian Hamster IgG
Concentration:
0.2 mg/mL
Conjugate:
PE-Cyanine7
Host Species:
Armenian Hamster
Target Species:
Not Applicable
Gene Alias:
IgG; Immunoglobulin G; ImmunoglobulinG
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
eBio299Arm
Applications:
Control, Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG
Purification Method:
Affinity chromatography
Antigen: MHC Class II (I-A/I-E)
Concentration: 0.2 mg/mL
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Alias: Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: M5/114.15.2
Applications: Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Antigen:
MHC Class II (I-A/I-E)
Concentration:
0.2 mg/mL
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
M5/114.15.2
Applications:
Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Antigen: FR4
Concentration: 0.2 mg/mL
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Alias: 0910001L11Rik; C86255; Folate receptor 4; folate receptor 4 (delta); folate receptor 4 (delta) homolog; folate receptor 4, delta (putative); folate receptor delta; Folate-binding protein 3; Folbp3; Folr4; FR-delta; IZUMO1 receptor protein JUNO; IZUMO1 receptor, JUNO; Izumo1r; Juno; probable folate receptor delta; sperm-egg fusion protein Juno
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: eBio12A5
Applications: Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Antigen:
FR4
Concentration:
0.2 mg/mL
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
0910001L11Rik; C86255; Folate receptor 4; folate receptor 4 (delta); folate receptor 4 (delta) homolog; folate receptor 4, delta (putative); folate receptor delta; Folate-binding protein 3; Folbp3; Folr4; FR-delta; IZUMO1 receptor protein JUNO; IZUMO1 receptor, JUNO; Izumo1r; Juno; probable folate receptor delta; sperm-egg fusion protein Juno
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
eBio12A5
Applications:
Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Antigen: OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Concentration: 0.2 mg/mL
Conjugate: PE-Cyanine7
Host Species: Mouse
Target Species: Mouse
Gene Alias: H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: eBio25-D1.16 (25-D1.16)
Applications: Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Antigen:
OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Concentration:
0.2 mg/mL
Conjugate:
PE-Cyanine7
Host Species:
Mouse
Target Species:
Mouse
Gene Alias:
H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
eBio25-D1.16 (25-D1.16)
Applications:
Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography