7091028

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Isotype Control

Manufacturer: Fischer Scientific

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Antigen

Mouse IgG1 kappa

Concentration

0.2 mg/mL

Conjugate

APC-eFluor 780

Host Species

Mouse

Target Species

Not Applicable

Gene Alias

IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183

Primary or Secondary

Isotype Control (Primary)

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

P3.6.2.8.1

Applications

Control, Flow Cytometry

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Isotype

IgG1 κ

Purification Method

Affinity chromatography

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Description

  • Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control
  • Applications Reported: The Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis
  • Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
  • Use isotype control at the same concentration as experimental antibody
  • APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Light sensitivity: This tandem is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
  • Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
  • It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
  • Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
  • It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
  • Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
  • It is also used in therapeutic applications, such as immunotherapy and vaccine development.

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7091028

Mouse Isotype Control...


Antigen:
Mouse IgG1 kappa

Concentration:
0.2 mg/mL

Conjugate:
APC-eFluor 780

Host Species:
Mouse

Target Species:
Not Applicable

Gene Alias:
IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
P3.6.2.8.1

Applications:
Control, Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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7091030

Mouse Monoclonal Antibody...


Antigen:
EOMES

Concentration:
5 μL/Test

Conjugate:
APC-eFluor 780

Host Species:
Mouse

Target Species:
Human, Porcine

Gene Alias:
C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
WD1928

Applications:
Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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7091031

Rat Monoclonal Antibody...


Antigen:
MHC Class II (I-A/I-E)

Concentration:
0.2 mg/mL

Conjugate:
APC-eFluor 780

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
M5/114.15.2

Applications:
Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

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7091032

Rat Monoclonal Antibody...


Antigen:
CD326 (EpCAM)

Concentration:
0.2 mg/mL

Conjugate:
APC-eFluor 780

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
adenocarcinoma-associated antigen; CD326; cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; EPCAM; Ep-CAM; EpCAM1; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; gp40; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; Ly74; lymphocyte antigen 74; M1S2; M4S1; major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; Protein D5.7A; Tacsd1; Tacstd1; TROP1; Trop-1 protein; Tumor-associated calcium signal transducer 1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
G8.8

Applications:
Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography