Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin
• Applications Reported: Rat IgG1 Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis
• Applications Tested: eFluor® 450 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes
• Use isotype control at the same concentration as experimental antibody
• eFluor® 450 is an alternative to Pacific Blue®
• eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics
• These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants
• Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens
• It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens
• Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases
• With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.