7111253
Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 702, eBioscience™, Invitrogen™
Mouse Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Mouse IgG2a kappa
Concentration
0.2 mg/mL
Conjugate
Super Bright 702
Host Species
Mouse
Target Species
Not Applicable
Gene Alias
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
eBM2a
Applications
Control, Flow Cytometry
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Related Products
Description
- The isotype of a primary antibody and the application it is being used in can result in background staining
- Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
- Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
- While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
- Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
- If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody
- Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
- It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
- Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
- It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
- Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
- It is also used in therapeutic applications, such as immunotherapy and vaccine development.
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Antigen: Mouse IgG2a kappa
Concentration: 0.2 mg/mL
Conjugate: Super Bright 702
Host Species: Mouse
Target Species: Not Applicable
Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary: Isotype Control (Primary)
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: eBM2a
Applications: Control, Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Antigen:
Mouse IgG2a kappa
Concentration:
0.2 mg/mL
Conjugate:
Super Bright 702
Host Species:
Mouse
Target Species:
Not Applicable
Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
eBM2a
Applications:
Control, Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Antigen: NK1.1
Concentration: 0.2 mg/mL
Conjugate: Super Bright 645
Host Species: Mouse
Target Species: Mouse
Gene Alias: AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein NKR-P1B allele B6; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele SJL/BALB; natural killer cell surface protein NKR-P1B allele TO; natural killer cell surface protein NKR-P1F; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; Nk1; Nk-1; NK1.1; Nk1.2; Nk-1.2; NKRP1; NK-RP1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1-b; NKR-P1B protein; Nkrp1c; NKR-P1C; Nkrp1d; NKR-P1D; Nkrp1f; NKR-P1F; RGD:2975}; UniProtKB:Q8VD98}
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: PK136
Applications: Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Antigen:
NK1.1
Concentration:
0.2 mg/mL
Conjugate:
Super Bright 645
Host Species:
Mouse
Target Species:
Mouse
Gene Alias:
AI462337; CD161; CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; EMBL:AAQ11375.1}; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; killer cell lectin-like receptor subfamily B member 1F; KLRB1; Klrb1b; klrb1b {ECO:0000312; Klrb1c; Klrb1d; Klrb1f; klrb1f {ECO:0000250; Ly55; Ly-55; Ly55b; ly-55b; Ly55c; ly-55c; Ly55d; Ly-55d; Ly59; Ly-59; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein NKR-P1B allele B6; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele SJL/BALB; natural killer cell surface protein NKR-P1B allele TO; natural killer cell surface protein NKR-P1F; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; Nk1; Nk-1; NK1.1; Nk1.2; Nk-1.2; NKRP1; NK-RP1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1-b; NKR-P1B protein; Nkrp1c; NKR-P1C; Nkrp1d; NKR-P1D; Nkrp1f; NKR-P1F; RGD:2975}; UniProtKB:Q8VD98}
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
PK136
Applications:
Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
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