Human IgE Isotype Control (HE1), Invitrogen™

Description

• The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody
• DIA HE1-01 contains purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma
• Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B, but must be handled as potentially infectious, as should all human material.The purity of this human IgE is >;98%, tested by SDS-PAGE
• As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes
• The human IgE is purified by Protein L chromatography
• The remaining contaminants are mainly components from Foetal Bovine Serum.This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation
• Other applications include immunochemistry and cellular immunology research.NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/ml