IL-5 Monoclonal Antibody (TRFK5), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5)
• The TRFK5 antibody is a neutralizing antibody
• Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE
• IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF)
• IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation
• In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production
• Applications Reported: The TRFK5 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-5 bioactivity, and IHC
• Applications Tested: The Functional Grade Purified TRFK5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-5 bioactivity
• The TRFK5 antibody at 0.06 μg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-5 in an MC/9 cell proliferation assay
• Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section
• IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils
• IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation
• The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes
• The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF)
• IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5
• IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.