Perforin Monoclonal Antibody (dG9 (delta G9)), APC, eBioscience™, Invitrogen™

Description

• Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp)
• Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK)
• Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections
• Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Perforin is one of the major cytolytic proteins of cytolytic granules
• Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules
• Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes
• Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD
• Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated
• Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested
• Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis
• Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood
• Alternative splicing results in multiple transcript variants of perforin.