5379796

Human B7-1/CD80 Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 9 publications

Manufacturer: Fischer Scientific

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Antigen

B7-1/CD80

Concentration

LYOPH

Applications

Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF, In Situ Hybridization (ISH), ELISA

Conjugate

Unconjugated

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias

Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80

Gene Symbols

CD80

Isotype

IgG1

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone

37711

Dilution

Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization 0.2-1 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Gene Accession No.

P33681

Gene ID (Entrez)

941

Immunogen

S. frugiperdainsect ovarian cell lineSf21-derived recombinant human B7-1/CD80 Val35-Asn242 (predicted) Accession # P33681

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.

Description

  • B7-1/CD80 Monoclonal specifically detects B7-1/CD80 in Human samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF-ready, Dual RNAscope ISH-IHC, ELISA Capture (Matched Antibody Pair).