5379796

Human B7-1/CD80 Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 9 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

B7-1/CD80

Concentration

LYOPH

Applications

Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF, In Situ Hybridization (ISH), ELISA

Conjugate

Unconjugated

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias

Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80

Gene Symbols

CD80

Isotype

IgG1

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone

37711

Dilution

Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization 0.2-1 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Gene Accession No.

P33681

Gene ID (Entrez)

941

Immunogen

S. frugiperdainsect ovarian cell lineSf21-derived recombinant human B7-1/CD80 Val35-Asn242 (predicted) Accession # P33681

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.

Description

  • B7-1/CD80 Monoclonal specifically detects B7-1/CD80 in Human samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF-ready, Dual RNAscope ISH-IHC, ELISA Capture (Matched Antibody Pair).

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Img

Fischer Scientific

5379796

Mouse Monoclonal Antibody has been ...


Antigen:
B7-1/CD80

Concentration:
LYOPH

Applications:
Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF, In Situ Hybridization (ISH), ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80

Gene Symbols:
CD80

Isotype:
IgG1

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone:
37711

Dilution:
Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization 0.2-1 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P33681

Gene ID (Entrez):
941

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human B7-1/CD80 Val35-Asn242 (predicted) Accession # P33681

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.

Img

Fischer Scientific

5379797

Mouse Monoclonal Antibody has been ...


Antigen:
B7-2/CD86

Concentration:
LYOPH

Applications:
Western Blot, Flow Cytometry, Neutralization, CyTOF, KnockDown

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

Gene Symbols:
CD86

Isotype:
IgG1

Purification Method:
Protein A or G purified from ascites

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone:
37301

Dilution:
Western Blot 5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.5-2.5 ug/mL, CyTOF-ready, Knockout Validated

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P42081

Gene ID (Entrez):
942

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human B7-2/CD86 Ala23-His244 Accession # P42081

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.

Img

Fischer Scientific

5379797

Mouse Monoclonal Antibody has been ...


Antigen:
B7-2/CD86

Concentration:
LYOPH

Applications:
Western Blot, Flow Cytometry, Neutralization, CyTOF, KnockDown

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

Gene Symbols:
CD86

Isotype:
IgG1

Purification Method:
Protein A or G purified from ascites

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone:
37301

Dilution:
Western Blot 5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.5-2.5 ug/mL, CyTOF-ready, Knockout Validated

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P42081

Gene ID (Entrez):
942

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human B7-2/CD86 Ala23-His244 Accession # P42081

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.

Img

Fischer Scientific

5379797

Mouse Monoclonal Antibody has been ...


Antigen:
B7-2/CD86

Concentration:
LYOPH

Applications:
Western Blot, Flow Cytometry, Neutralization, CyTOF, KnockDown

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene Alias:
Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86

Gene Symbols:
CD86

Isotype:
IgG1

Purification Method:
Protein A or G purified from ascites

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Clone:
37301

Dilution:
Western Blot 5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.5-2.5 ug/mL, CyTOF-ready, Knockout Validated

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P42081

Gene ID (Entrez):
942

Immunogen:
S. frugiperdainsect ovarian cell lineSf21-derived recombinant human B7-2/CD86 Ala23-His244 Accession # P42081

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.