7068364
IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IL-10
Applications
Flow Cytometry
Conjugate
FITC
Host Species
Rat
Target Species
Mouse
Gene Alias
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
Gene Symbols
IL10
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
IL10
Clone
JES5-16E3
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Gene Accession No.
P18893
Gene ID (Entrez)
16153
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10)
- Mouse IL-10 is an ∽18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF)
- In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10
- IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response
- Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity
- Applications Tested: This JES5-16E3 antibody has been pre-titrated and tested by intracellular staining for flow cytometric analysis
- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood cells
- This can be used at test size: 5 μL (0.25 μg)/test per test
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 μm post-manufacturing filtered
- IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
- This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
- It also enhances B cell survival, proliferation, and antibody production
- Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
- Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
- Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
- Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.