Description

• General description: 1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.[1][2]
• Application: N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.[3][4]
• Quality: Purity: 99% (from C), homogeneous (GC) Ease of removal*: ++CMC**: 14.5 mM at +25°CFormula: C14H28O6
• Preparation Note: Working concentration: 46 mMWorking solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.Storage conditions (working solution): Stability in SolutionA stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.
• Storage and Stability: Store at 15 to 25 °C. (Store dry!)
• Other Notes: For life science research only. Not for use in diagnostic procedures.