Description

• CHAPSO, or 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate, is a zwitterionic detergent commonly used in biochemical and biophysical research
• It is widely employed for membrane protein solubilization, stabilization, and purification
• CHAPSO is known for its mildness and ability to preserve the structural integrity and functionality of membrane proteins during extraction
• Its unique properties make it suitable for a variety of applications, including protein crystallization, spectroscopy, and structural biology studies
• With its versatile and effective solubilization capabilities, CHAPSO is a valuable tool for researchers working with membrane proteins
• Features of CHAPSO include: • Zwitterionic detergent • Gentle—enabling preservation of native conformation and activity • Effectively disrupts lipid-protein interactions • Exhibits low critical micelle concentration (CMC) • High-purity compound with low UV absorptivity Properties • CMC: (H 2 O) ~8 mM (0.50%) • Aggregation number: (H 2 O) ~11 • Purity: ≥99% (by HPLC analysis) • pH: 5–8 (1% solution in water) • Solubility: ≥0.5 M (in water at 20 ̊C) • Fluorescence: <10% (0.1% solution in water at 345 nm) • Absorbance of a 1% solution in water: 260 nm: <0.06, 280 nm: <0.04, 340 nm: <0.02