Gibco™ ExpiFectamine™ 293 Transfection Kit

Description

• Description The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System
• It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells
• The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System Provides robust and reproducible transfection results, giving users greater confidence in their results Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System
• The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2
• The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression
• The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.