Invitrogen™ Lens Culinaris (Edible Lentil) Agglutinin (LCA), fluorescein (FITC)

Description

• LCA specificity is similar to concanavalin A, but narrower, resulting in differences in patterns of tissue staining
• Wheat germ agglutinin, concanavalin A, and LCA are known to block inhibitory effects of nerve growth factor in cell-free systems
• • Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm • Applications: immunofluorescence, glycobiology • Sugar specificity: mannose, glucose • Inhibiting/eluting sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside • Blood group specificity: non-specific • Unconjugated lectin molecular weight: 50 KDa • Immunofluorescence working concentration: 5–20 μg/mL LCA-FITC is provided at a 5 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca 2+
• If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.