ENG Scientific Leifson Bacterial Flagella Stain Kit

Description

• Kit Includes Solution I - Basic Fuchsin, (1.2%) Alcoholic 250 mL, Solution II - Tannic Acid (3%) 250 mL, Solution III - Sodium Chloride (1.5%) 250 mL, and Solution IV - Methylene Blue 250 mL Bacterial flagella are below the visual limit in size, approximately 0.1 to 0.3 μ in diameter
• Flagella stains increase the width of these delicate appendages by forming a precipitate at the surface of the flagella making them visible with the optical microscope
• The preparation may be used to establish polar or lateral distribution of flagella on a bacterial cell
• Recommended Procedure: Preparation of Slides: Slides must be clean and grease free
• To assure this, soak slides for 4 days in a slide cleaning solution (Product No
• 6981)
• Thoroughly wash slides in distilled water and allow to dry
• Working Solution: Mix equal parts of each solution (I, II, III) in a glass vessel and tightly stopper
• Allow to stand 2 hours prior to use
• NOTE: Working stain solution will stain flagella satisfactorily for only 2 - 3 days; therefore, only prepare as needed
• This solution precipitates rapidly at room temperature
• Use only the clear supernatant for staining
• From a centrifuged 24 hour broth culture of a motile species of bacteria, a loopful of sediment is placed at the end of the slide
• Tilt the slide allowing the liquid to run slowly to the other end and air dry
• Quickly add 1 mL of stain mixture
• Allow to stand 5 - 15 minutes at room temperature
• When precipitate forms over the entire area of the slide, carefully wash off by flooding with water
• Counterstain with 1:10 dilution of Sol
• IV for 1 minute
• Wash and drain dry
• Results: Flagella stain bright red
• Contents: Basic Fuchsin, Ethanol, Tannic Acid, Sodium Chloride, Methylene Blue, HCl These solutions are made from certified dyes (when applicable)
• For in vitro diagnostic use
• 1 Kit