ES7150
ENG Scientific Pneumocystis Carinii Stain Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ES7150-Each-of-1 | In Stock | ₹ 45,945.72 |
ES7150 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,945.72
GST (18%): ₹ 8,270.23
Total Price: ₹ 54,215.95
Type
Modified Toluidine Blue O Stain
Quantity
4 x 250 mL
Includes
250 mL each of Solutions I, II, III and IV
For Use With (Application)
In vitro diagnostic use
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Description
- Pneumocystis carinii is an opportunist which causes a diffuse interstitial pneumonia in patients with impaired immune systems
- The disease itself can be classified as epidemic or sporadic; the latter occurring in patients who have an underlying immunosuppression
- In this procedure, a sulfation reagent of glacial acetic acid and sulfuric acid (not included) is used for the removal of background material
- This allows the Pneumocystis carinii cysts to be visualized more easily after staining with Toluidine Blue 0
- Diagnosis of Pneumocystis carinii pneumonia can frequently be made on bronchoalveolar lavage (BAL) or on touch preparations of pulmonary tissue (open lung biopsies and transbronchial biopsies)
- Recommended Procedures: BAL Specimens: Using a 50 mL tube, centrifuge for 15 minutes at 2000 x g
- Aspirate all but the bottom 5 mL of supernatant
- With a Pasteur pipette, aspirate the sediment plus approximately 1 mL of the remaining 5 mL of fluid
- Transfer material to a 15 mL centrifuge tube, gently mix and prepare smear by spreading a drop over a 1 cmm area
- If concentrated specimen is very thick or mucoid, spread over entire slide with care being taken not to make the smear too thick
- Dry the slides on a heating block at 50 - 55°C
- Allow to cool before staining
- Touch Preparations: When dry, process in same manner as slides of BAL
- Preparation of Sulfation Reagent: In a dry coplin jar submerged in a plastic tub filled with cool water (not below 10°C), mix 45 mL of glacial acetic acid with 15 mL of concentrated sulfuric acid and stir gently with glass rod
- Carefully place smears in reagent for 10 minutes
- Mix immediately and again after 5 minutes
- Gently rinse with water for 10 minutes
- Drain excess water and stain with Sol
- I for 3 - 5 minutes
- Rinse with Sol
- II followed by Sol
- III for 10 seconds each
- Clear in Sol
- IV, two changes each for 10 seconds and mount
- Results: The cyst forms appear as lavender structures (cup-shaped) approximately 5 urn in diameter
- The cyst outline is distinct, and the internal region stains uniformly
- Note: A negative bronchoalveolar lavage does not rule out an infection with Pneumocystis carinii
- Other diagnostic procedures may be necessary.
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Show Difference
Type: Modified Toluidine Blue O Stain
Quantity: 4 x 250 mL
Includes: 250 mL each of Solutions I, II, III and IV
For Use With (Application): In vitro diagnostic use
Type:
Modified Toluidine Blue O Stain
Quantity:
4 x 250 mL
Includes:
250 mL each of Solutions I, II, III and IV
For Use With (Application):
In vitro diagnostic use
Type: Ethanol 95%
Quantity: 2 x 250 mL
Includes: Toluidine Blue O Stain
For Use With (Application): In vitro diagnostic use
Type:
Ethanol 95%
Quantity:
2 x 250 mL
Includes:
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For Use With (Application):
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Type: Xylene
Quantity: 2 x 250 mL
Includes: Solution IV - Xylene
For Use With (Application): In vitro diagnostic use
Type:
Xylene
Quantity:
2 x 250 mL
Includes:
Solution IV - Xylene
For Use With (Application):
In vitro diagnostic use
Type: Gram-Weigart Stain Kit
Quantity: 6 x 100 mL
Includes: 100 mL each of Solutions I, II, III, IV and V
For Use With (Application): Pneumocystis carinii
Type:
Gram-Weigart Stain Kit
Quantity:
6 x 100 mL
Includes:
100 mL each of Solutions I, II, III, IV and V
For Use With (Application):
Pneumocystis carinii