Thermo Scientific™ Remel™ BactiDrop Calcofluor White

Description

• Detect fungi, yeasts, Microsporidia, Acanthamoeba, and Pneumocystis carinii using Thermo Scientific™ Remel™ BactiDrop™ Calcofluor White fluorescent stain
• In 1961, Darken observed the uptake of calcofluor white by actively growing cultures of yeast and higher fungi1
• Hageage and Harrington used calcofluor white (CFW) to demonstrate hyphae and yeasts in clinical specimens2
• Baselski and Robison reported the use of CFW to detect P
• carinii cysts3
• Wilhelmus et al
• demonstrated the fluorescence of Acanthamoeba keratitis4
• Weber et al
• reported CFW also stains microsporidia spores5
• Calcofluor White is a rapid, non-specific, fluorochrome stain used in microscopic detection of fungi, yeasts, Acanthamoeba cysts, microsporidia, and Pneumocystis carinii in clinical specimens
• Recently calcofluor white has been used to detect fungi in direct preparations and in deparaffinized tissue sections6,7
• CFW is a chemofluorescent optical brightening agent that has the ability to bind with cellulose and chitin
• This compound delineates the cell walls of cellulose containing organisms upon excitation with longwave ultraviolet light2.Ready-to-use: Liquid reagentEasy to interpret:Fungal elements, Yeast - Bright apple green fluorescence with typical morphologyAcanthamoeba cysts - Cysts (10-25 μm) appear corrugated and doublewalled with bright apple-green fluorescence and bright orange cytoplasm.Microsporidia - Apple-green fluorescence, intestinal microsporidia spores range in size from 0.9-1.5 μm to 1.2-2.0 μm, cell wall is brightened but staining is not specific.Pneumocystis carinii - Brilliant apple-green fluorescence, cysts are 5-8 μm in diameter and contain up to 8 crescent or pleomorphic shaped sporozoites, cell wall and double parenthesis structures inside the cysts stain intensely.Bacteria - Weak to no fluorescence, typical coccoid or bacillary shapeThis reagent contains calcofluor white (1g/1000mL) as the fluorescent stain
• Evans blue dye is added to minimise background material
• Before staining with CFW, potassium hydroxide 10% (REF R21524) is used as a clearing agent to dissolve tissue cells.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.