Thermo Scientific™ Gram Stain Kit

Description

• Differentiate gram-positive organisms from gram-negative organisms using Thermo Scientific™ Remel™ Gram Stain Kit
• The Gram stain method was developed in 1884 by the Danish bacteriologist, Christian Gram, to differentiate bacterial cells from infected tissue 1
• Later it was discovered that the bacterial cell wall composition was the key to the Gram stain and would differentiate bacteria into two groups based on cell color after staining
• Since that time there have been many modifications of the original technique 2,3
• Use of gram staining kit helps in differentiating gram-positive organisms from gram-negative organisms
• Ready to use: Liquid reagents supplied in 250mL bottles Ease of identification: As it stains the gram positive organisms as purple and gram-negative organisms as red Rapid results: 5-10 mins for the staining The gram staining kit consists of 4 bottles containing 250 mL of Gram Crystal Violet, Safranin, Gram Iodine and Gram Decolorizer each
• Crystal Violet, a basic dye, is the primary stain taken up by all bacteria due to its ability to rapidly permeate the cell wall
• It stains the protoplast of bacteria purple
• The potassium-iodine mixture, in gram iodine, is the mordant which complexes with the primary stain in the cell
• In gram-positive cells, the crystal violet-iodine complex is trapped in the cell due to a decrease in cell wall permeability caused by alcohol dehydration
• Gram-positive cells are stained purple
• In gram-negative cells, the complex is removed by the Decolorizer due to an increase in permeability caused by solubility of the lipids in alcohol
• The counterstain used must be a contrasting color to the primary stain and in this case it is Safranin, which stains gram-negative cells red
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.