Thermo Scientific™ Phenolic Acridine Orange

Description

• Detect acid-fast bacilli in clinical specimens with Thermo Scientific™ Remel™ Phenolic Acridine Orange
• In 1977, Kronvall and Myhre described the use of acridine orange stain to detect microorganisms in direct smears prepared from clinical specimens 1
• In 1955, Smithwick et al
• described an acid-fast method which incorporated an acridine orange solution that contained phenol 2
• Phenolic Acridine Orange stain was found to produce less nonspecific fluorescence of background material
• This makes it superior to auramine O
• Phenolic Acridine Orange is a fluorescent stain recommended for use with Phenolic Acridine Orange Decolorizer (REF R40181) for detection of acid-fast bacilli in clinical specimens
• Phenolic Acridine Orange stain is a dye which binds to the nucleic acid of bacteria and other cells, either in the native or the denatured state
• Phenol enhances penetration of acridine orange through the mycobacterial cell wall
• Ready-to-use – Liquid reagent Easy-to-observe - Examine smears with a fluorescent microscope with 200 X magnification and confirm the morphology with 600 X magnification
• Easy-to-interpret - Positive test shows bright-red to orange fluorescent bacilli with characteristic morphology confirmed at 600 X magnification
• In case of negative test no bright-red to orange fluorescent bacilli are observed after examination of 30 fields at 200 X magnification
• Phenolic Acridine Orange contains acridine orange (CAS 10127-02-3) as the dye, glycerol (CAS 56-81-5) which increases the viscosity of the solution and keeps the stain on the smear during the staining procedure, and phenol crystals (CAS 108-95-2) to enhance penetration of the dye through the mycobacterial cell wall
• It also contains 95% ethyl alcohol (CAS 64-17-5) and demineralized water (CAS 7732-18-5) to make up the solution
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.