Promega RNasin™ Ribonuclease Inhibitor: Natural
Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.
Manufacturer: Fischer Scientific
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Concentration
20 to 40 U/μL
Formulation
Amount of RNasin™ RNase inhibitor required to inhibit the activity of 5ng of RNase A by 50%, activity is measured by the inhibition of hydrolysis of Cytidine 2,3'-cyclic Monophosphate by RNase A
For Use With (Application)
RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems, performing RNase-free Antibody staining, enhancement of RNA yields from Riboprobe™ system RNA synthesis reactions
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Description
- Broad-Spectrum Inhibition of Common Eukaryotic RNases Native, recombinant and oxidation-resistant forms available Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase
- Use in many downstream assays: Inhibits a broad spectrum of eukaryotic RNases over a wide pH range (pH 5-8) Maintains inhibitory activity over a wide temperature range
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Concentration: 20 to 40 U/μL
Formulation: Amount of RNasin™ RNase inhibitor required to inhibit the activity of 5ng of RNase A by 50%, activity is measured by the inhibition of hydrolysis of Cytidine 2,3'-cyclic Monophosphate by RNase A
For Use With (Application): RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems, performing RNase-free Antibody staining, enhancement of RNA yields from Riboprobe™ system RNA synthesis reactions
Concentration: __
Formulation: __
For Use With (Application): Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
Concentration: __
Formulation: Tryptone 10g/L, Yeast extract 5g/L, NaCl 10g/L
For Use With (Application): For growth of bacteria