Description

• General description: 1-Deoxynojirimycin is a polyhydroxypiperidine alkaloid and glucose analog, that has an -NH group substituting the O atom in the pyranose ring. It shows a significant α-glucosidase inhibitory effect in addition to antioxidant, antimicrobial, antidiabetic, antitumor, and anti-inflammatory behavior. It is commonly found in the roots of mulberry trees.[1][1]
• Application: DNJ may be used as a reference standard for the determination of DNJ in: Mulberry leaves by high-performance liquid chromatography (HPLC) equipped with evaporative light scattering detector (ELSD)[2] as well as direct analysis in real-time (DART) ionization source coupled with triple quadrupole tandem mass spectrometry (MS/MS).[3]Silkworms by hydrophilic interaction chromatography (HILIC) with MS/MS operating on multiple reaction monitoring (MRM) mode.[4] Mulberry leaves of 132 varieties belonging to nine Morus species following its derivatization with fluorenylmethyl chloroformate (FMOC-Cl) by high-performance liquid chromatography (HPLC) combined with photodiode array (PDA) detectorDevelopment of an analytical method based on hydrophilic interaction chromatography (HILIC) coupled with tandem mass spectrometry (MS/MS) in positive ion electrospray ionization mode in the leaves of 32 cultivars of different Morus speciesHPLC method-based separation and quantification in the ethanol extracts of the leaves of Morus alba L. and Morus nigra with fluorimetric detection following pre-column derivatization with 9-fluorenylmethyl chloroformate146 varieties of mulberry fruits from nine genera by HPLC in combination with UV-Vis dual-wavelength detectionHuman plasma by hybrid quadrupole/linear ion trap tandem mass spectrometry (QTRAP MS/MS).[5]