Thermo Scientific Chemicals Denhardt's solution (50X)

Description

• Denhardt's solution is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes
• Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols
• This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio
• Some documentation and label information may refer to the legacy brand
• The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals
• General Description • Formulated in 1966, Denhardt’s solution is a combination of high-molecular weight polymers used to prevent non-specific binding of nucleic acid probes to membranes and filters • Denhardt’s solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin Application • Denhardt’s solution is used in Northern and Southern blotting to prevent non-specific binding of detection reagents to membranes • It can be used along with gelatin-chrome alum in in situ hybridization assays to maintain chromosome integrity • It has been used to facilitate quantitative DNA hybridization on nylon membranes