Thermo Scientific Chemicals TRIS-glycine-SDS running buffer (10X), pH 8.3

Description

• Tris-Glycine SDS Running Buffer (10X) is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE)
• It is ideally suited for protein gel analysis in biochemical, molecular biological, cell biological,and immunological research studies
• This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio
• Some documentation and label information may refer to the legacy brand
• The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals
• General Description • TRIS-glycine-SDS running buffer (10X), is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins Application • This solution is the most commonly used buffer for protein gel analysis in several research studies • It is also used to block membranes with 5% non-fat dry milk for western blotting • TRIS-glycine-SDS running buffer is commonly used as both anode buffer and cathode buffer • The use of SDS in the buffer helps keep the proteins linear
• Glycine is an amino acid whose charge state plays an important role in the stacking gel