Thermo Scientific™ Pierce™ HeLa Digest/PRTC Standard

Description

• The Thermo Scientific Pierce HeLa Digest/PRTC Standard is a validated complex mammalian protein digest containing additional PRTC heavy isotope-labeled peptides designed for LC/MS performance testing
• Features of HeLa Digest/PRTC Standard include: Convenient —pre-mixed formulation of HeLa digest and PRTC standard in a ready-to-use format Informative —enables robust performance assessment of LC/MS instruments Verified —high-quality, consistent mixture documented via lot-specific certificates of analysis Stable —provided in a lyophilized format The Pierce HeLa Digest/PRTC Standard is a lyophilized mixture of 10 μg of HeLa protein digest with 5 pmol PRTC peptides to assess chromatography MS instrument performance
• The protein digest is derived from a well-established adenocarcinoma reference HeLa S3 cell line that expresses over 15,000 proteins with relevant post-translational modifications
• This complex digest is an ideal standard for complex proteome mass spectrometry applications
• The included Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy isotope-labeled peptides mixed at an equimolar ratio that elute across the chromatographic gradient
• The observed retention times and hydrophobicity factors (HFs) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship
• This equation and the HF of an uncharacterized peptide are then used to predict retention time
• In combination, the mixture is a powerful tool to optimize and assess LC parameters, identify the total peptide elution window, and optimize and monitor MS instrumentation
• This standard is specifically formulated for LC/MS experiments and does not contain salts or detergents
• Using the standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time
• Applications: Qualitative LC assessment QC assay tool for assessment of LC/MS system performance LC/MS standardization LC/MS method development and optimization Normalize results for variation in retention times and peak intensities between runs