For optimal stability, store at -80°C Keep Frozen Repeated freeze-thaws will destroy product integrity Innovative approach to modeling the morphological effects of oncogenesis and development using three-dimensional microenvironments Gelling: Basement Membrane Extract forms a hydrogel that supports cell proliferation, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test Thaw 3-D Culture Matrix BME Coated 96 Well Plate at Room Temperature for one hour, and then transfer to a 37°C cell culture incubator for 30 minutes Cell number may be determined using 3D Culture Proliferation Core Kit Plate provides a convenient, standardized, physiologically predictive format for evaluating the pharmacological effects of compounds for these cell culture models.