Promega CellTox™ Green Cytotoxicity Assay

Description

• Assay system measures changes in membrane integrity that occur as result of cell death
• It is intended to assess cytotoxicity in cell culture after experimental manipulation
• It uses proprietry asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells
• When dye binds DNA released from cells, its fluorescence properties are substantially enhanced
• Viable cells produce no appreciable increases in fluorescence
• Therefore, fluorescence signal produced by binding interaction with dead cell DNA is proportional to cytotoxicity
• Binds DNA of cells that have lost membrane integrity throughout 72-hour exposure Dye can be diluted in culture medium and delivered directly to cells at seeding or at dosing or diluted in assay buffer and delivered to cells as endpoint measure after exposure period Can scale from 96- to 1536-well plate formats with “no-addition” or “single-addition” protocols Available as CellTox Green Cytotoxicity Assay and CellTox Green Express Cytotoxicity Assay kits Lysis solution also available separately CellTox Green Cytotoxicity Assay 100μL/assay in 96-well plate or 25μL/assay in 384-well plate format Follows Endpoint Assay Protocol, 2X reagent addition Kit contains green dye, assay buffer and lysis solution CellTox Green Express Cytotoxicity Assay For 96-well plate or 384-well plate format when used at recommended final dilution of 1:1,000 in assay well Includes 200μL Green dye