R&D Systems™ Human CD3+ T Cell Enrichment Column, Mini

Description

• The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain
• The CD3 complex functions to transduce intracellular signals during TCR antigen recognition
• CD3 is expressed early in thymocyte development, and on a subset of NK cells
• CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction
• The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents Total CD3+ cell recovery from CD3+ cells loaded ranges between 32% and 40% Purity of recovered cells ranges between 85% and 95% These enriched T cell populations are then available for tissue culture, activation studies, tissue typing, immune status monitoring, and flow cytometry