4922979
Invitrogen™ Rhod-2, AM, cell permeant
Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
Manufacturer: Fischer Scientific
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Content And Storage
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application)
Cell Viability and Proliferation
Product Type
Stain
Shipping Condition
Room Temperature
Detection Method
Fluorescence
For Use With (Equipment)
Fluorescence Microscope
Dye Type
Fluorescent Dye-Based
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Description
- Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca 2+
- They have uses in many calcium signaling investigations, including measuring Ca 2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca 2+ release generated by photoreceptors and photoactivatable chelators
- Fluorescence signal from these cells is generally measured using fluorescence microscopy Label (Ex/Em of Ca 2+ -bound form): Rhod-2 (552/581nm) Fluorescence intensity increase upon binding Ca 2+ : >100 fold K d for Ca 2+ in the absence of Mg 2+ , in buffer: approximately 570nM Exhibit fluorescence increase upon binding Ca 2+ with little shift in wavelength In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn 2+ , Zn 2+ , Pb 2+ ) with substantially higher affinity than Ca 2+ Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling Order Info Shipping Condition: Room temperature
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Content And Storage: Store in freezer -5°C to -30°C and protect from light.
For Use With (Application): Cell Viability and Proliferation
Product Type: Stain
Shipping Condition: Room Temperature
Detection Method: Fluorescence
For Use With (Equipment): Fluorescence Microscope
Dye Type: Fluorescent Dye-Based
Content And Storage:
Store in freezer -5°C to -30°C and protect from light.
For Use With (Application):
Cell Viability and Proliferation
Product Type:
Stain
Shipping Condition:
Room Temperature
Detection Method:
Fluorescence
For Use With (Equipment):
Fluorescence Microscope
Dye Type:
Fluorescent Dye-Based
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Content And Storage: Store at 4°C.
For Use With (Application): Transfection
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Store at 4°C.
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Transfection
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