Invitrogen™ DAF-FM Diacetate (4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate)

Description

• It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole
• DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers
• Ex/Em of DAF-FM: approx
• 495/515nm Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution DAF-FM diacetate is cell permeant and passively diffuses across cellular membranes; once inside the cell, it is converted to cell-impermeant for Buffers containing bovine serum albumin (BSA) and phenol red may affect fluorescence and should be used with caution Fluorescence quantum yield of DAF-FM is approx
• 0.005, but increases about 160-fold, to ∼0.81, after reacting with NO Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation in vivo imaging of NO in zebrafish I ntravital microscopic detection of NO generation associated with angiogenesis in mice Quantitation of ATP-induced NO release in rabbit platelets Spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5 NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM approx
• 3nM versus approx
• 5nM for DAF-2) Refer to the Molecular Probes™ Handbook for additional product information
• Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitric Oxide Research, Nitro-Oxidative Stress Order Info Shipping Condition: Room Temperature