Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520

Description

• Fixable Viability Dyes may be used to label cells from all species Fixable Viability Dye eFluor™ 520 can be excited by the blue (488nm) laser line and has a peak emission of 522nm that can be detected using a 530/30 band pass filter (equivalent to FITC) For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
• After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
• Fixable Viability Dye eFluor™ 520 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
• It should be protected from light and moisture Store at −70°C with dessicant It may be freeze-thawed up to 20 times Allow vial to equilibrate to room temperature before opening Staining may be done before or after surface staining Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing