Molecular Probes™ Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry

Description

• Easily differentiate live, dead, and apoptotic cells during flow cytometry with our Dead Cell Apoptosis kits with Annexin V conjugates including Alexa Fluor 488, FITC, propidium iodide, PE, APC, and SYTOX Green
• These conjugates distinguish live, dead, or apoptotic cells via different dyes, which is vital for confirming cell viability and apoptosis using multi-parametric studies
• There are several advantages to using Dead Cell Apoptosis kits with Annexin V for flow cytometry in order to assay cell viability: Superior brightness Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells
• The Alexa Fluor 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC)
• High binding efficiency Annexin V conjugates are made from a highly purified cys-annexin, resulting in higher binding efficiency and extremely accurate characterization of the apoptotic process
• Multi-parametric Many publications require a minimum of two different ways to identify apoptotic cells
• This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane, while membrane integrity is determined via propidium iodide
• Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & PI The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & Propidium Iodide (PI) (V13241, V13245) is used in flow cytometry to measure early apoptosis by detecting phosphatidyl serine (PS) expression and membrane permeability
• When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence
• Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence
• Dead Cell Apoptosis Kit with Annexin V PE and SYTOX Green The Dead Cell Apoptosis Kit with Annexin V PE and SYTOX Green detects PS externalization in apoptotic cells during flow cytometry using recombinant annexin V conjugated to the orange fluorescent phycobiliprotein R-PE, while dead cells are detected with SYTOX Green nucleic acid stain
• After treatment with both probes, apoptotic cells show orange fluorescence, dead cells show green fluorescence, and live cells show little or no fluorescence
• These populations can easily be distinguished in the 530/30 nm and 585/42 nm bandpass filters with a 488 nm laser flow cytometer
• Dead Cell Apoptosis Kit with Annexin V APC and SYTOX Green The Dead Cell Apoptosis Kit with Annexin V APC and SYTOX Green detects PS externalization in apoptotic cells during flow cytometry using recombinant annexin V conjugated to red laser-excited allophycocyanin, and dead cells using SYTOX Green nucleic acid stain
• Apoptotic cells are detected by annexin V binding to externalized PS
• Live cells show little or no fluorescence, apoptotic cells show red fluorescence and very little green fluorescence, and late apoptotic cells show a higher level of red and orange fluorescence
• These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser)
• The Dead Cell Apoptosis Kit with Annexin V FITC and PI The Dead Cell Apoptosis Kit with Annexin V FITC and PI detects PS externalization in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using PI
• Necrotic cells that stain with PI show red fluorescence
• After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.