Invitrogen™ eBioscience™ Indo-1 AM Calcium Sensor Dye (UltraPure Grade)

Description

• Unbound Indo-1 has a peak emission at 475nm Upon binding calcium, peak emission shifts down to 410nm In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 475nm; bound Indo-1 fluorescence should be collected using a filter below 400nm Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eVolve™ 605 or eVolve™ 655 is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1 Molecular weight: 1009.91g/mol Peak excitation: 346nm Peak emission: 475nm Reconstitute in high-quality, freshly opened DMSO Recommended concentration: 1 mM Once reconstituted, store at −20°C with dessicant and use within a short period of time
• Avoid freeze-thawing